Fig. 1: MLN4924 upregulates MICA and MICB expression in human MM cell lines. | Cell Death & Disease

Fig. 1: MLN4924 upregulates MICA and MICB expression in human MM cell lines.

From: Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition

Fig. 1

MICA and MICB cell surface expression was analyzed by flow cytometry on SKO-007(J3), RPMI-8226 and U266 cells treated with MLN4924 (50–400 nM) for 72 h. Representative overlays are shown. Histograms represent the average of the mean fluorescence intensity (MFI) values of the indicated ligand (with treatment-specific isotype control MFI subtracted out). The MFI of MICA and MICB were calculated based on at least four independent experiments and statistical significance (±SEM) was evaluated by paired Student t test (*P < 0.05).

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