Fig. 1: MYCN promotes nucleotide synthesis. | Cell Death & Disease

Fig. 1: MYCN promotes nucleotide synthesis.

From: Therapeutic targeting of both dihydroorotate dehydrogenase and nucleoside transport in MYCN-amplified neuroblastoma

Fig. 1: MYCN promotes nucleotide synthesis.

A Schematic of pyrimidine biosynthesis via de novo and salvage pathways with indicated fold changes in mRNA expression of the pathway enzymes determined by microarray. B Bar plot of KEGG metabolic processes upregulated by MYCN via transcriptional activation. C qRT-PCR analysis of mRNA expression for enzymes involved in de novo nucleotide synthesis in non-MYCN-amplified cell lines with inducible MYCN expression in the absence of doxycycline (Doxy) for six days. Data are mean ± SD (n = 4). P values were determined by two-tailed Student’s t-test. ***P < 0.001, n.s., not significant. D Relative levels of intracellular nucleotides in non-MYCN-amplified SHEP1 cells with constitutive MYCN expression (MYCN) in comparison with vector-control cells (pCDH). Data are mean ± SD (n = 3). P values were determined by Welch’s t-test. **P < 0.01, ***P < 0.001.

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