Fig. 5: Physiological concentrations of uridine antagonize the action of DHODH inhibitors. | Cell Death & Disease

Fig. 5: Physiological concentrations of uridine antagonize the action of DHODH inhibitors.

From: Therapeutic targeting of both dihydroorotate dehydrogenase and nucleoside transport in MYCN-amplified neuroblastoma

Fig. 5: Physiological concentrations of uridine antagonize the action of DHODH inhibitors.

A Cell-growth assay of MYCN-amplified cell lines treated with GSK983 in the presence of increasing concentrations of supplemental uridine (Urd) in culture media containing 10% FBS for four days. Data are mean ± SD (n = 4). P values were determined by two-tailed Student’s t-test between Urd-0 and individual Urd-containing groups. **P < 0.01, ***P < 0.001. B, C Dose–response curves for GSK983 (B) and BRQ (C) in MYCN-amplified cell lines cultured in the presence of 10% or 50% FBS for four days. Data are mean ± SD (n = 4). D Dose-response curves for GSK983 in MYCN-amplified cell lines cultured in the presence of 10% or 50% dialyzed FBS for 4 days. Data are mean ± SD (n = 4). E Dose–response curves for GSK983 in MYCN-amplified cell lines cultured in the presence of 10% or 50% FBS before and after dialysis for four days. Data are mean ± SD (n = 4). F Dose–response curves for GSK983 in IMR5 cells cultured in media containing 50% dialyzed FBS without or with supplemental uridine or nucleosides for four days. Data are mean ± SD (n = 4).

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