Fig. 2: LPS-induced ferroptosis results in a negative effect on the immune function of DCs.

DCs were respectively pretreated with Era (20 μM) and Lip-1 (1 μM) 1 h prior to LPS stimulation. A Percentages of co-stimulatory phenotypes expressed on DCs. B–E Supernatant levels of IL-12, IL-6, IL-1β, and TNF-α in cultured DCs were detected by ELISA. F–G CD4 ⁺T cells stained with Con A were incubated with DCs at a ratio of 1:200. Levels of IFN-γ and the IFN-γ/IL-4 ratio were examined by ELISA to evaluate Th1/Th2 polarization. H Stimulated DCs were cocultured with CD4⁺ T cells incubated with Con A, and then T-cell proliferation induced by DCs was evaluated by flow cytometry. The results were shown as the as mean ± SD (n = 3 in each group). Statistical significance: *P < 0.05 for the WT-Era group vs. the WT-control group; ^#P < 0.05 for the WT-Lip-1 group vs. the WT-Era group; ^&P < 0.05 vs. the WT-LPS group.