Fig. 4: The protective effect of Sesn2 on DCs is related to the inhibition of ferroptosis.

DCs obtained from splenocytes of WT mice and Sesn2^−/− mice, were pretreated with Lip-1 (1 μM) 1 h prior to LPS stimulation. A Expression of co-stimulatroy phenotypes was determined in murine DCs treated with LPS or pretreated with Lip-1 in advance of LPS. B–E Secretion levels of inflammatory cytokines in supernatants from stimulated DCs were assessed by ELISA. F–G CD4⁺ T cells incubated with Con A were cocultivated with DCs at a ratio of 1:200. Levels of IFN-γ and the IFN-γ/IL-4 ratio were evaluated by ELISA. H Proliferation of CD4⁺ T cells was examined by CFSE staining after stimulation for 48 h with DCs pretreated with LPS or Lip-1. Values of three independent experiments were expressed as the mean ± SD (n = 3 per group). Statistical significance: *P < 0.05, **P < 0.01 vs. the control group.