Fig. 6: Sesn2 downregulates sepsis-induced ferroptosis to protect the immune response of DCs.

Mice were intraperitoneally preinjected with Lip-1 at 10 mg/kg and underwent severe CLP 1 h later. Mice in the control group underwent a sham operation. A The percentage of co-stimulatory phenotypes expressed on DCs was measured by flow cytometry. B–E The release of inflammatory cytokines reflecting the degree of DC maturity and secretion was detected by ELISA after CLP. F, G DCs extracted in CLP with injection of Lip-1 or without Lip-1 were cocultured with CD4⁺ T cells incubated with Con A. Afterwards, IFN-γ contents and IFN-γ/IL-4 ratios were determined by ELISA to evaluate Th1/Th2 polarization. H DCs from CLP mice treated with or without Lip-1, were cocultured with CD4⁺ T cells stained with Con A, and then T-cell proliferation induced by DCs was measured by flow cytometry. I Mice were treated with Lip-1 at a concentration of 10 mg/kg and underwent severe CLP 1 h later. Data from three independent experiments were presented as the mean ± SD (n = 3 per group). Statistical significance: *P < 0.05, **P < 0.01 vs. the control group.