Fig. 1: Expression landscape and clinical correlation of RNA m5C regulators in gastric cancer (GC) and NSUN2.

A A heatmap was used to visualize the expression of m5C regulators in TCGA-STAD database. Red and blue regions represent higher and lower expression levels, respectively. B Interactions between m5C regulators in GC. The size of each circle represents the influence of each regulator on prognosis, as determined via the log-rank test. The lines connecting regulators represent their interactions, and the thickness of the line represents the correlation strength. A positive correlation is marked with blue and a negative correlation with red. The regulator clusters A–C were marked red, blue, and green, respectively. C Expression of NSUN2 across 33 different cancers in TCGA database. D Representative images of immunohistochemical staining for NSUN2 protein in a tissue microarray (×40: scale bar = 100 μm; ×400: scale bar = 10 μm) and (E and F) the differential expression of NSUN2 between tumor and adjacent normal tissue. G Kaplan–Meier OS analysis of NSUN2 expression in GC patients. *P < 0.05, **P < 0.01, and ***P < 0.001.