Fig. 5: Effect of morpholino on suppression of aberrant splicing of Pms2 c.1993A>G transcript. | Cell Death & Disease

Fig. 5: Effect of morpholino on suppression of aberrant splicing of Pms2 c.1993A>G transcript.

From: A novel mouse model of PMS2 founder mutation that causes mismatch repair defect due to aberrant splicing

Fig. 5

A Schematic representation of the use of morpholino to block the de novo splice site in Pms2 transcript with c.1993A>G mutation in exon 11. Uppercase letters mark the exon sequence and lowercase letters represent intronic sequence. 5 bases that are encoded by exon 11 and deleted in the transcript due to c.1993A>G mutation are marked in blue. B Chromatogram showing the restoration of WT splice site in Pms2ki/ki mouse embryonic fibroblast (MEF) cells. MEFs were transfected either with 10 μM control or Pms2 specific morpholino. Arrow marks the presence of c.1993A>G mutation in Pms2 transcript.

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