Fig. 2: LPCAT1 promotes ESCC cell proliferation, migration, and invasion. | Cell Death & Disease

Fig. 2: LPCAT1 promotes ESCC cell proliferation, migration, and invasion.

From: LPCAT1 reprogramming cholesterol metabolism promotes the progression of esophageal squamous cell carcinoma

Fig. 2

A–D PCR and western blotting showing the expression of LPCAT1 in EC9706 cells (A, B) and TE1 cells (C, D) transfected with si-NC and si-LPCAT1. Data are shown as relative expression means and P value from three independent experiments. E, F The proliferative ability of EC9706 cells (E) and TE1 cells (F) after transfection was evaluated using CCK-8 assay. G, H Colony-formation assay for LPCAT1-knockdown EC9706 cells and TE1 cells (G) and quantitative analysis of LPCAT1 in each group (H). I, J Transwell chamber assays for LPCAT1-depletion ESCC cells. The average numbers of migrated cells were counted after 24 h incubation and expressed as mean ± S.D. K, L Matrigel invasion assays for LPCAT1-depletion ESCC cells. The average numbers of migrated cells were counted after 24 h incubation and expressed as mean ± S.D. Data are from three independent experiments. *P < 0.05, **P < 0.01 (one-way ANOVA).

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