Fig. 5: LPCAT1 promote ESCC cholesterol synthesis by EGFR/INSIG-1/SREBP-1 pathway.

A The level of cholesterol in EC9706 and TE1 cells transfected with sh-control and sh-LPCAT1 was detected using the cholesterol detection kit. B The expression of SQLE, Insig-1, and MSMO1 in EC9706 and TE1 cells transfected with sh-control and sh-LPCAT1 was detected using western blot. C, D The expression of p-SREBP-1 in the nucleus in EC9706 and TE1 cells transfected with sh-control and sh-LPCAT1 was detected using western blot and immunofluorescence. E The expression of p-EGFR and p-PI3K in EC9706 and TE1 cells transfected with sh-control and sh-LPCAT1 was detected using western blot. F The expression of SQLE, Insig-1, and MSMO1 in EC9706 and TE1 cells treated with NSC228155 or 740Y-P after transfection with sh-LPCAT1 was detected using western blot. G The expression of p-SREBP-1 in the nucleus in EC9706 and TE1 cells treated with NSC228155 or 740Y-P after transfection with or without sh-LPCAT1 was detected using western blot. H The level of cholesterol in EC9706 and TE1 cells treated with NSC228155 or 740Y-P after transfection with or without sh-LPCAT1 was detected using the cholesterol detection kit. Data represent three independent experiments. *P < 0.05, **P < 0.01 (unpaired t test, one-way ANOVA). Data are from three independent experiments.