Fig. 6: Acetylation of PDHE1α is increased in tubules from fibrotic kidneys.

A Cellular component GO enrichment analysis of upregulated acetylated proteins in tubules separated from UUO and sham-operated mice. Bubble chart shows top 20 significantly enriched terms (vertical axis, functional category; horizontal axis, Log2 transformed ratio of upregulated acetylated proteins to identified proteins. Colored circles indicate significant p values; circle size indicates a number of differential proteins in terms. B, C Western blots of phosphorylated PDHE1α and total PDHE1α expression in tubules from UUO and sham-operated mice on POD 1. PDHE1α served as the standard (*P < 0.05; n = 3). D Enzyme activity of PDH in tubular mitochondria from UUO and sham-operated mice on POD 1 (*P < 0.05 vs. sham; n = 4); E, F Western blots of phosphorylated PDHE1α and total PDHE1α expression in tubules from UUO and sham-operated mice injected with or without HKL on POD 1. PDHE1α served as the standard (*P < 0.05, ^†P < 0.05; n = 3). G Enzyme activity of PDH in tubular mitochondria from UUO and sham-operated mice injected with or without HKL on POD 1. (*P < 0.05, ^†P < 0.05; n = 5–6). H, I Western blots of phosphorylated PDHE1α and total PDHE1α expression in tubules from Sirt3 KO and WT mice with UUO on POD 1. PDHE1α served as the standard (*P < 0.05; n = 3). J Enzyme activity of PDH in tubular mitochondria from Sirt3 KO and WT mice with UUO on POD 1. (*P < 0.05; n = 6).