Fig. 5: PP2Ac is necessary for PDCD10-mediated YAP activation.

A The protein-protein interaction network of PDCD10 was constructed in STRING database (http://string-db.org). B GSEA of PDCD10 associated genes in TCGA HCC database showed that PDCD10 was associated with protein phosphatase 2A binding and phosphatase activity. C The enzymatic activity of PP2Ac in Hep3B^shPDCD10, HepG2^PDCD10 and their control cells. Data are the mean ± SD of three independent experiments. D Co-immunoprecipitation assay was carried out to determine the direct binding between PDCD10 and PP2Ac in Hep3B and HepG2^PDCD10 cells. E Double immunofluorescence staining showed the colocalization of PDCD10 and PP2Ac in Hep3B cells. Scale bars, 5 μm. F Co-immunoprecipitation analysis of PP2Ac and YAP in Hep3B^shPDCD10, HepG2^PDCD10 and their control cells. G Double luciferase reporter assays showed the TEAD transcriptional activity in PDCD10-interfered HCC cells with PP2Ac knockdown or ectopic expression. Data are the mean ± SD of three independent experiments. H Western blot analysis of p-YAP, YAP, CTGF and CYR61 in PDCD10-interfered HCC cells with PP2Ac knockdown or ectopic expression. I In vitro dephosphorylation assay was performed to test the effect of PDCD10 on dephosphorylation ability of PP2Ac. **P < 0.01; ***P < 0.001.