Fig. 4: Zinc affects the enzyme activity, protein and mRNA levels of the two Mmps. | Cell Death & Disease

Fig. 4: Zinc affects the enzyme activity, protein and mRNA levels of the two Mmps.

From: Fear-of-intimacy-mediated zinc transport controls fat body cell dissociation through modulating Mmp activity in Drosophila

Fig. 4

A Gelatin zymogram revealed that total Mmp enzyme activity was considerably diminished in Lsp2-Gal4 > foi RNAi larvae while increased in Lsp2-Gal4 > foi OE larvae. B Quantitative measurement of (A). C–F Protein levels of both Mmp1 and Mmp2 in the fat body were reduced in Lsp2-Gal4 > foi RNAi larvae while increased in Lsp2-Gal4 > foi OE larvae. Dietary zinc supplementation was able to elevate the expression of Mmps, whereas zinc chelator TPEN can reduce their expression. G, H Both Mmp1 and Mmp2 mRNA levels were significantly down-regulated in Lsp2-Gal4 > foi RNAi larvae, whereas a dramatically up-regulated Mmp2 mRNA level could be detected in Lsp2-Gal4 > foi OE larvae. w1118 flies driven by Lsp2-Gal4 serve as a blank control. All the experiments were conducted independently at least for 3 times. ns: not significant, *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed Student’s t test.

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