Fig. 6: FGF21 upregulated autophagy flux by enhancing the TFEB activity. | Cell Death & Disease

Fig. 6: FGF21 upregulated autophagy flux by enhancing the TFEB activity.

From: Fibroblast growth factor 21 (FGF21) alleviates senescence, apoptosis, and extracellular matrix degradation in osteoarthritis via the SIRT1-mTOR signaling pathway

Fig. 6: FGF21 upregulated autophagy flux by enhancing the TFEB activity.

A–C Western blotting revealed the levels of cytoplasmic TFEB and nuclear TFEB in chondrocytes as treated above. D, E Western blotting revealed the levels of the autophagic proteins (p62 and LC3II) and Lysosomal protein (Lamp1) in chondrocytes as treated above. F, G The mRFP-GFP-LC3 assay was performed to detect the number of autophagosomes in chondrocytes (yellow signal represents autophagosomes; red signal represents autophagolysosomes; scale bar: 10 μm). The data in the figures are the averages ± S.D. Significant differences between the treatment and control groups are indicated as **P < 0.01 and *P < 0.05, n = 3.

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