Fig. 3: RANBP10 recovery restored the cell proliferation, migration, and invasion of RANBP10-knockdown GBM cells.
From: RANBP10 promotes glioblastoma progression by regulating the FBXW7/c-Myc pathway
A Western blot and RT-PCR analysis were performed to detect the expression of RANBP10 in the indicated cells. B, C MTT assays were performed to examine the effect of RANBP10 overexpression on the proliferation of RANBP10-knockdown and control cells. D BrdU assays were performed to examine the effect of RANBP10 overexpression on the DNA synthesis ability of RANBP10-knockdown and control cells. Scale bar = 50 μm. E Transwell assays were used to detect the effect of RANBP10 overexpression on the migration of RANBP10-knockdown and control cells. Scale bar = 50 μm. The data were expressed as mean ± SD. Student’s t test was performed to analyzed significance. *P < 0.05, **P < 0.01, ***P < 0.001.
