Fig. 5: TMEM97 potentiates the interaction of LRP6 with CK1δ/ε. | Cell Death & Disease

Fig. 5: TMEM97 potentiates the interaction of LRP6 with CK1δ/ε.

From: Transmembrane protein 97 exhibits oncogenic properties via enhancing LRP6-mediated Wnt signaling in breast cancer

Fig. 5

A HEK293T cells were transfected with the vectors expressing CK1δ-V5 (A) or CK1ε-V5 (B) alone or combined with TMEM97-Flag expression vector. Cell lysates were immunoprecipitated with anti-V5 beads. Immunoblotting was carried out using the indicated antibodies. C–D HEK293T cells were transfected with TMEM97-Flag expression plasmid. Cell lysates were immunoprecipitated with IgG or anti-CK1δ (C) or anti-CK1ε (D) antibodies. Immunoblotting was performed using the indicated antibodies. E Cell lysates from MDA-MB-231 cells were subjected to immunoprecipitation with IgG or anti-CK1δ antibody. Immunoblotting was performed using the indicated antibodies. The images shown are representative of data generated in at least three independent experiments.

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