Fig. 6: TMEM97 knockout attenuates the Wnt/β-catenin signaling cascade through regulating LRP6 phosphorylation at Ser1490 in breast cancer cells. | Cell Death & Disease

Fig. 6: TMEM97 knockout attenuates the Wnt/β-catenin signaling cascade through regulating LRP6 phosphorylation at Ser1490 in breast cancer cells.

From: Transmembrane protein 97 exhibits oncogenic properties via enhancing LRP6-mediated Wnt signaling in breast cancer

Fig. 6

A The expression of TMEM97 protein was detected by immunoblot analysis in normal mammary epithelial Hs578Bst cells and breast cancer Hs578T, MCF-7, MDA-MB-468, MDA-MB-231, BT549, and SW527 cells. B Knockout of TMEM97 downregulated the Wnt/β-catenin pathway via reducing LRP6 phosphorylation at Ser1490 in Hs578T and MDA-MB-231 cells. C Knockout of TMEM97 decreased the mRNA expression of Wnt target genes AXIN2, LEF1, survivin, and fibronectin in Hs578T and MDA-MB-231 cells. Data represent the mean ± SD (n = 3, *P < 0.05). D Western blot analysis of the protein expression of Wnt target genes AXIN2, LEF1, and survivin in Hs578T and MDA-MB-231 cells with TMEM97 knockout. The images shown are representative of data generated in at least three independent experiments.

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