Fig. 1: LASP1 is associated with sphingomyelin metabolism and regulates ECHS1 in CRC cells. | Cell Death & Disease

Fig. 1: LASP1 is associated with sphingomyelin metabolism and regulates ECHS1 in CRC cells.

From: ECHS1, an interacting protein of LASP1, induces sphingolipid-metabolism imbalance to promote colorectal cancer progression by regulating ceramide glycosylation

Fig. 1

A GSEA of the top 100 upregulated LASP1-related pathways, including biocarta ceramide, PID_S1P_S1P2 and PID_S1P_META. B Heatmap of the LC–MS assay for RKO-LASP1 and control cells. C Heat map depicting the differentially expressed lipomics for RKO-LASP1 and control cells. D Histogram of c. The asterisk (*) indicates P < 0.05. The double asterisk (**) indicates P < 0.01. The quadra asterisk (****) indicates P < 0.0001. E Fluorescence images show the two differentially expressed protein spots in DIGE analysis. The interacting protein spots are indicated (white arrows). F MS of in-gel trypsin digests of the protein and analysis of the depicted peptide spectrum resulted in the identification of ECHS1. G, H RT-PCR (G) and Western blotting (H) were performed to detect the expression of ECHS1 and LASP1 in LASP1-overexpressing and LASP1-knockdown cells. I Representative figures and data of the Transwell assay for LV-control-, LV-LASP1-, and LV-LASP1 plus siRNA-ECHS1-transfected RKO cells. Bars in the right panel represent the number of migrated and invaded cells.

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