Fig. 4: The effect of vezatin knockdown on AMPAR- and NMDAR-mediated synaptic currents in the hippocampal CA1 neurons of epileptic mice.

a Representative images of AMPAR-mEPSCs in the Scr-seq group and shRNA group and (b) the corresponding statistical analysis of the amplitude (presented as medians and ranges; P = 0.003; Kruskal–Wallis test; **P < 0.01) and frequency of AMPAR-mEPSCs (n = 9 per group). c Representative images of NMDAR-mEPSCs in the Scr-seq group and shRNA group and (d) the corresponding statistical analysis of the amplitude (presented as medians and ranges; Kruskal–Wallis test) and frequency of NMDAR-mEPSCs (n = 9 per group). Current–voltage (I–V) relationships obtained by plotting the amplitudes of AMPAR-eEPSCs at holding potentials ranging from −60 mV to +40 mV in the Scr-seq group (e) and in the shRNA group (f). The normalized I–V curves of AMPAR-eEPSCs (normalized to the amplitude evoked at −60 mV) (g) and statistical analysis of the Scr-seq group and shRNA group (h). The RI was calculated from the I–V curves as the ratio of the AMPAR-eEPSC amplitude at −60 mV to the AMPAR-eEPSC amplitude at +40 mV (n = 8 per group; P < 0.001; Student’s t test; ***P < 0.001).