Fig. 6: Numb regulates Notch signaling by binding to NICD in vitro. | Cell Death & Disease

Fig. 6: Numb regulates Notch signaling by binding to NICD in vitro.

From: Loss of Numb promotes hepatic progenitor expansion and intrahepatic cholangiocarcinoma by enhancing Notch signaling

Fig. 6

A Proliferation, metastasis, and stemness were measured by colony formation, migration, wound-healing, and sphere formation assays after DAPT treatment at a dose of 150 μM (the control group was treated with the same volume of DMSO without DAPT) in HuCCT1 cells. Scale bar, 200 μm. B Immunofluorescence staining of HPC markers (CD133 and Sox9) in HuCCT1 cells. Scale bars, 50 μm (CD133) and 100 μm (Sox9). C Immunofluorescence staining of the key proteins of Notch signaling, NICD, and Hes1, in HuCCT1 cells. Scale bar, 50 μm. D Western blotting displaying the upregulation of HPC markers and the downstream factors of Notch after Numb silencing was reversed after DAPT treatment in HuCCT1 cells. E Coimmunoprecipitation (CoIP) showing the direct binding of Numb and NICD. All data represent the mean ± SD of at least three independent experiments; *P < 0.05, **P < 0.01, and ***P < 0.001.

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