Fig. 6: Inhibition of autophagy ameliorates TGF-β1 induced kidney fibrosis and apoptosis in HK-2 cells.
From: Reciprocal regulation between ER stress and autophagy in renal tubular fibrosis and apoptosis
HK-2 cells were treated with or without TGF-β1 (5 ng/ml) for 24 h in the absence or presence of CQ (20 μM) for 24 h. A Cell lysates were analyzed for LC3 and β-actin by immunoblot analysis. B Densitometric analysis of LC3-II band signal. C Representative phase-contrast and TUNEL staining images. Scale bar = 100 μm. D Counting of TUNEL staining positive cells. E Cell lysates were analyzed for FN, Collagen I, and cleaved Caspase-3 by Immunoblot analysis. F Densitometric analysis of FN and cleaved Caspase-3 immunoblots relative to β-actin. G Representative images of Collagen I immunofluorescence staining. Scale bar = 100 μm. H Quantitative analysis of Collagen I immunofluorescence intensity. Data are expressed as mean ± SD. n = 4. *p < 0.05; **p < 0.01; ***p < 0.001.
