Fig. 1: Knockdown of KTN1 mediates post-transcriptional reduction of EGFR protein expression, increases apoptosis, and represses survival of CSCC cells. | Cell Death & Disease

Fig. 1: Knockdown of KTN1 mediates post-transcriptional reduction of EGFR protein expression, increases apoptosis, and represses survival of CSCC cells.

From: Kinectin1 depletion promotes EGFR degradation via the ubiquitin-proteosome system in cutaneous squamous cell carcinoma

Fig. 1

A HSC-1 cells were transfected with siNC, siKTN1 or siKTN1#, and total RNA were extracted and analyzed by qRT-PCR. qRT-PCR results represent the means ± SD. Representative of results from three biological replicates. B HSC-1 cells were transfected with siNC, siKTN1, siKTN1#, or co-transfected with siKTN1 and KTN1mut, total proteins were extracted and analyzed by immunoblotting. Quantified results represent the means ± SD. Representative of results from three biological replicates. C HSC-5 cells were transfected with siNC, siKTN1, or siKTN1#, and total RNA were extracted and analyzed by qRT-PCR. qRT-PCR results represent the means ± SD. Representative of results from three biological replicates. D HSC-5 cells were transfected with siNC, siKTN1, siKTN1#, or co-transfected with siKTN1 and KTN1mut, total proteins were extracted and analyzed by immunoblotting. Quantified results represent the means ± SD. Representative of results from three biological replicates. E HaCaT cells were transfected with siNC, siKTN1, or siKTN1#, and total RNA were extracted and analyzed by qRT-PCR. qRT-PCR results represent the means ± SD. Representative of results from three biological replicates. F HaCaT cells were transfected with siNC, siKTN1, siKTN1#, and co-transfected with siKTN1 and KTN1mut, total proteins were extracted and analyzed by immunoblotting. Quantified results represent the means ± SD. Representative of results from three biological replicates. G, H A431 and HSC-5 cells were transfected with siNC, siEGFR-1, siEGFR-2, or co-transfected with siEGFR-1 and EGFRmut, and total protein was extracted and analyzed by immunoblotting. Quantified results represent the means ± SD. Representative of results from three biological replicates. I A431 cells were transfected with siNC, siKTN1, siKTN1#, and co-transfected with siKTN1 and KTN1mut, total proteins were extracted and analyzed by immunoblotting. Quantified results represent the means ± SD. Representative of results from three biological replicates. J Flow cytometry analysis of apoptotic cells in A431 and HSC-5 cells transfected with siNC, siKTN1, siKTN1#, or co-transfected with siKTN1 and KTN1mut. K Apoptotic cell ratios of A431 and HSC-5 cells transfected with siNC, siKTN1, siKTN1#, or co-transfected with siKTN1 and KTN1mut. Apoptotic cell ratios represent the means ± SD. Representative of results from three biological replicates. L, M Colony formation assay and survival fractions of A431 and HSC-5 cells transfected with siNC, siKTN1, siKTN1#, or co-transfected with siKTN1 and KTN1mut. Survival fractions represent the means ± SD. Representative of results from three biological replicates.

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