Fig. 8: In vivo function of the EGFR degradation regulatory axis triggered by KTN1. | Cell Death & Disease

Fig. 8: In vivo function of the EGFR degradation regulatory axis triggered by KTN1.

From: Kinectin1 depletion promotes EGFR degradation via the ubiquitin-proteosome system in cutaneous squamous cell carcinoma

Fig. 8

A, B Tumorigenicity of KTN1 depletion and control A431 cells in nude mice. siKTN1-transfected or siNC-transfected A431 cells (0.1 mL; 2 × 106 cells) were injected into different sides of nude mice. Tumor growth was measured every 2 days. Two weeks later, the mice were sacrificed to collect the tumor samples. Photo credit: Ji Ma, Department of Radiation medicine, School of Public Health, Southern Medical University. Tumor volumes represent the means ± SD. C Representative photos of immunohistochemistry (IHC) staining with anti-PSMA1–7, anti-ADRM1, anti-EGFR, anti-CCDC40, and anti-KTN1 for the xenograft tumor model. D Lysates of the xenografted tumors were subjected to immunoblotting with anti-PSMA1–7, anti-ADRM1, anti-EGFR, anti-CCDC40, or anti-KTN1. E–H Representative photos and integrated optical densities of immunohistochemistry (IHC) staining with anti-PSMA1–7, anti-ADRM1, and anti-CCDC40 for normal human tissues and human cSCC tissues. Data represent the means ± SD. I Lysates of normal human tissues and human cSCC tissues were subjected to immunoblotting with anti-PSMA1-7, anti-ADRM1, or anti-CCDC40.

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