Fig. 4: Loss of CDK8 in TNBC enhances NK-cell-mediated tumor surveillance. | Cell Death & Disease

Fig. 4: Loss of CDK8 in TNBC enhances NK-cell-mediated tumor surveillance.

From: Triple-negative breast cancer cells rely on kinase-independent functions of CDK8 to evade NK-cell-mediated tumor surveillance

Fig. 4

A Experimental setup of data shown in (B–E). Rag2−/− mice were orthotopically transplanted with E0771 control, shCdk8-1, or shCdk8-2 cells (in independent experiments) and monitored for primary tumor growth. Tumors were surgically removed and regrowth and distant metastasis formation of tumor cells was monitored with in vivo imaging. B In vivo primary tumor growth for control vs. shCdk8-1 or shCdk8-2 E0771 cells in the left and right panel, respectively. C Total infiltrating NK-cell numbers (CD45+NK1.1+NKp46+) of digested primary tumors at day of surgery. D Radiance signals of lungs ex vivo measured by an in vivo-imaging system and representative pictures of lungs (lower panel) at the end of the experiment (day 23 post surgery). E Total numbers of infiltrating NK cells (CD45+NK1.1+NKp46+) of lung lysates were assessed. Symbols and error bars represent mean ± SEM (n = 4–9 mice per group).

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