Fig. 4: Specific contribution of the necroptosis-pathway to MVA-induced cell death. | Cell Death & Disease

Fig. 4: Specific contribution of the necroptosis-pathway to MVA-induced cell death.

From: Diversity of cell death signaling pathways in macrophages upon infection with modified vaccinia virus Ankara (MVA)

Fig. 4

a, b D7 differentiated wt (black bars) or RIPK3−/− Hoxb8 macrophages (gray bars) were infected with MVA at an MOI of 2 and harvested after 22 h by accutase treatment followed by annexin/PI staining (a) or active caspase-3 staining (b) and flow cytometry analysis. c, d Bax/Bak-RIPK3-TKO (light gray bars) or Bax/Bak-MLKL TKO macrophages (dark gray bars), genetically modified by CRISPR/Cas9, were infected with MVA at an MOI of 2 and harvested after 22 h by accutase treatment. Cells were subjected to annexin/PI staining (c) or active caspase-3 staining (d) followed by flow cytometry analysis. Bax/Bak-DKO ctrl cells expressing an irrelevant gRNA directed against EGFP served as control (black bars). e, f D7 differentiated wt (black bars), Caspase-1−/− (gray bars), or Caspase-1/11 DKO Hoxb8 macrophages were infected with MVA at an MOI of 2 and harvested after 22 h by accutase treatment followed by annexin/PI staining (e) or active caspase-3 staining (f) and flow cytometry analysis. Inhibitors were used where indicated at the following concentrations: QVD, 20 µM; ZVAD, 50 µM; Nec-1, 10 µM. Shown are means/SEM of n = 4 (a, b) or n = 3 (c–e) independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns nonsignificant (p ≥ 0.05).

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