Fig. 3: ARID1A deficiency weakens BRG1-RAD21 axis via BRG1 suppression.

A Glycerol sedimentation assay with 5 to 35% gradients for detecting the co-segregation of Brg1 and Rad21 in nuclear fractions in Arid1a WT and KO AB17 cells. The dotted lines enclose the co-density stratifications of Brg1 and Rad21. B Sucrose sedimentation (5 to 50%) assay in Arid1a WT and KO AB17 cells. The co-fractionation of Brg1 and Rad21 circled with dotted lines. C, D The same as A and B respectively but in HCC cell line MHCC-97H. E HEK293T cells transfected with the T7-ARID1A construct exhibited no significant impact on CTCF and RAD21 (Left). Similarly, no impact on ARID1A in CTCF (Medium) and RAD21 over-expressed cells (Right). F Neither CTCF nor RAD21 was effected by ARID1A deficiency in AB17 (Left) and MHCC-97H cells (Right). G Brg1 mRNA expression levels in Arid1a WT and KO AB17 cells, Q506 cells, primary hepatocytes, MEFs (193-4 and 102-2 cells), and HCC cell line MHCC-97H with three biological replicates. Error bars were painted by SEM. p value was calculated by a two-tailed Student’s t-test. mRNA expression of Gapdh was employed as an internal control.