Fig. 2: Elevated LSD1 is important for the maintenance of CSC phenotypes in HNSCC. | Cell Death & Disease

Fig. 2: Elevated LSD1 is important for the maintenance of CSC phenotypes in HNSCC.

From: Targeting LSD1 suppresses stem cell-like properties and sensitizes head and neck squamous cell carcinoma to PD-1 blockade

Fig. 2: Elevated LSD1 is important for the maintenance of CSC phenotypes in HNSCC.The alternative text for this image may have been generated using AI.

A Representative coimmunofluorescence staining images for LSD1 and the CSC markers Bmi-1 and CD44 in HN12 cells (scale bar, 50 μm). B Representative images of colony formation and tumorsphere formation by HN4 and HN12 cells. Scale bar = 200 μm. C Measurement of colony formation and tumorsphere formation (mean ± SD from three separate experiments) by HN12 and HN4 cells. D An Aldefluor assay was conducted with HN4 and HN12 cells, and the percentage of ALDH-high cells was quantified by flow cytometry. E The expression of LSD1 and CSC-related proteins in HN4 and HN12 cells was detected by western blotting. F Western blot analysis of LSD1 in HN12 and HN30 cells stably expressing a control vector or LSD1-specific shRNA. G An ALDEFLUOR assay was conducted with HN12 LSD1-knockdown and control cells, and the percentage of ALDH-high cells was quantified by flow cytometry. H Images of tumorsphere formation by HN12 and HN30 cells stably expressing a control vector or LSD1-specific shRNA. Scale bar = 200 μm. I The graph demonstrates the mean ± SD for the tumorsphere number of HN12 cells stably expressing a control vector or LSD1-specific shRNA from three separate experiments. J The graph demonstrates the mean ± SD for the tumorsphere number of HN30 cells stably expressing a control vector or LSD1-specific shRNA from three separate experiments. K, L HN30 cells stably transfected with control or LSD1-specific shRNAs were injected into nude mice. Tumor growth was monitored every 3 days; tumor size and weight were recorded. The data are presented as mean ± SEM from five mice. *P < 0.05 vector control cells compared with corresponding LSD1-knockdown cells.

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