Fig. 5: MK2 activation is essential for p38α-SUMOylation to mediate OS response in GC cells.
a p38-associated proteins were detected by Western blotting in indicated cell lines generated from both MGC803 and HGC27. b The interaction between HA-p38αWT or D150A and MK2 was detected by co-immunoprecipitation analysis in MGC803/MAPK14KO cells. c MGC803 cell lysates were incubated with purified GST-p38αWT or p38α protein overnight at 4 °C and subjected to immunoblotting analysis. d–i ROS levels in indicated HGC27 stable cell lines after treatment with MK2-IN-1 hydrochloride (10 mM) for 24 h were determined by DCFH-DA probe (d–f) and CellRox Orange (g–i) for fluorescence microscopy (d, g) and flow cytometry analysis (e, h). The data are presented as mean ± S.D. **p < 0.01 (f, i).
