Fig. 5: Linc-RoR specifically regulates DEPDC1 by facilitating the interaction of HNRNPK with DEPDC1 mRNA.

a Venn diagram showing the RNA-binding protein shared in RBPDB, Starbase database, MS-protein and PMID:25365966. b The schematic diagram of the RNA pull-down method used to identify the binding between Linc-ROR and RNA-binding protein in both HepG2 and Hep3B cells; interaction of Linc-RoR with HNRNPK, as detected by RNA precipitation using Linc-RoR as an RNA probe, followed by western blot. c RIP assay using HNRNPK antibody confirms that Linc-RoR interacts with HNRNPK. d Increase of DEPDC1 mRNA stability in Lv-RoR cells as compared to control cells. Cells were treated with 2.5 μg/mL actinomycin D and RNA was isolated at 0, 4, 8 and 12 h, respectively. e Re-expression of Lv-shRoR restores its ability to decrease DEPDC1 mRNA stability. f The schematic diagram of the RNA pull-down method used to identify the binding between DEPDC1 and RBP in both HepG2 and Hep3B cells; interaction of DEPDC1 with HNRNPK, as detected by RNA precipitation using DEPDC1 as an RNA probe, followed by western blot. g RIP assay using HNRNPK antibody confirms that DEPDC1 interacts with HNRNPK. h Linc-RoR is required for the interaction of HNRNPK with DEPDC1 mRNA, as detected by RIP assay using HNRNPK antibody. i Linc-RoR enhances the interaction of DEPDC1 mRNA with HNRNPK, as detected by RNA precipitation using DEPDC1 3ʹ-UTR probe. Data are shown as mean ± SD, n = 3. The data statistical significance is assessed by Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001.