Fig. 3: Enrichment and protein–protein interaction analyses reveal vesicle and inflammation pathways enriched in the necroptotic EVs.

A Processes enriched in the TBQ EV-enriched proteins (Fisher’s exact test, FDR = 0.02, compared to the Vesiclepedia human proteome database). Color bars represent Fisher’s test p-values, symbol size represents intersection size between our dataset and the GO category. B Protein–protein interaction network of the TBQ EV-enriched proteins (based on the String database). Node size represents fold change of TBQ vs. control. Edge width represents protein–protein co-expression level. Marked in color are proteins belonging to selected enriched biological processes. EVs, extracellular vesicles; TBQ, TNF-α, Birinapant, and QVD-OPh; GO, gene ontology; FDR, false discovery rate.