Table 2 Target, dilutions, and concentrations of reagents used for staining.
From: Proteomic analysis of necroptotic extracellular vesicles
Reagent | Target | Permeability | Dilution/concentration | Manufacturer |
Propidium iodide (PI) | Nucleic acids | – | 1 μg/mL | Sigma-Aldrich (Merck Millipore) |
AnnexinV (A5) | Phosphatidylserine | – | 1:200 | MBL (Woburn, MA, USA) |
Carboxyfluorescein succinimidyl ester (CFSE) | Cellular amines (lysine residues) | + | 5 μM | Sigma‐Aldrich |
Hoescht33342 | Nucleic acids | + | 1 μg/mL | Sigma‐Aldrich |
