Fig. 2: C/EBPδ is induced by the PERK pathway of the unfolded protein response in MDA-MB-435S and KPL-4 cells. | Cell Death & Disease

Fig. 2: C/EBPδ is induced by the PERK pathway of the unfolded protein response in MDA-MB-435S and KPL-4 cells.

From: PERK signaling through C/EBPδ contributes to ER stress-induced expression of immunomodulatory and tumor promoting chemokines by cancer cells

Fig. 2

a–c qRT-PCR analysis of the indicated mRNA levels in MDA-MB-435S cells transfected with control siRNA (siNS) or a siATF6A (ATF6α), b siERN1 (IRE1α), or c siPERK (targeting EIF2AK3), and treated for 6 h with 100 nM Tg (+) or DMSO (−). d Western blot analysis of MDA-MB-435S cells transfected with siRNA and treated with Tg (100 nM) for 3 h as indicated. e–h Western blot analysis (e, g) and qRT-PCR analysis (f, h) of MDA-MB-435S (e, f) and KPL-4 (g, h) cells treated with DMSO or Tg (100 nM) for 3 h and with or without pre-treatment with 1 µM GSK2606414 (GSK-414). i qRT-PCR analysis of the indicated mRNA levels in MDA-MB-435S cells transfected with siNS or siATF4 and treated with DMSO or Tg for 6 h. Quantitative data are represented as mean ± S.E.M, n = 3; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 for comparisons to siNS or vehicle control *without Tg or #with Tg treatment, n.s., not significant.

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