Fig. 6: Potential regulation of KAT2A on cGAS in SLE.

Positive signals were shown as green fluorescence for cGAS, red for KAT2A, blue for DAPI (A); the obtained PBMCs from SLE group and healthy donors co-cultured with PBS or KAT2A inhibitor respectively. The expression level of cGAS pathway was detected by RT-PCR after co-culture for 24 h (B, C); the concentration of IFN-1 in the supernatant was detected by ELISA after co-culture (D); difference of detection indices after different treatment (co-cultured with PBS or KAT2A inhibitor) in the same sample (E−G). Samples from healthy donors or patients have inconsistent levels of this difference. *, **, *** and **** means p < 0.05, p < 0.01, p < 0.001 and p < 0.0001, respectively.