Fig. 2: Effect of GPER on the intestinal mucosal barrier in 5-FU induced CIM.
CIM model was induced by i.p. injection 5-FU (30 mg/kg/day) for 5 days. G-1 (0.03 mg/kg/day), G15 (0.3 mg/kg/day) were administrated intraperitoneally with 5-FU together. G-1 was administrated alone or with G15 in CIM model to test the effect of GPER activation. G15 was used alone to test the effect of endogenous GPER blocking. On the 5th day, the ileum was collected for immunofluorescence, immunohistochemical staining and western blot. Data were expressed as mean ± SEM (*P < 0.05, **P < 0.01, ***P < 0.001). a Immunofluorescence for ZO-1 in ileum to show the effect of G-1 on ZO-1 expression in the CIM model (scale bars: 50 μm). b Representative images for immunohistochemical staining of Muc-2 in ileum following G-1 administration with or without G15 in the CIM model (scale bars: 50 μm). c Representative western blots photographs for ZO-1 following G-1 administration with or without G15 in the CIM model. d Representative western blots photographs for ZO-1 following endogenous GPER blocking with G15 alone in the CIM model. e Statistical analysis of ZO-1 expression in ileum tissue within four subgroups to show the effect of GPER activation on ZO-1 expression in the CIM model (n = 4). f Statistical analysis of ZO-1 expression in ileum tissue within three subgroups to show the effect of endogenous GPER blocking on ZO-1 expression in the CIM model (n = 4). g Effect of GPER activation on the number of Muc-2+ cells per villus within four subgroups in the CIM model. At least 20 villi were counted randomly for each slide, and the mean value was calculated for single sample (n = 6). h Effect of GPER activation with G-1 on ileal mucosal permeability within four subgroups in the CIM model (n = 6).
