Fig. 4: Effect of GPER activation on crypt cell proliferation in 5-FU induced CIM. | Cell Death & Disease

Fig. 4: Effect of GPER activation on crypt cell proliferation in 5-FU induced CIM.

From: Activation of G protein coupled estrogen receptor prevents chemotherapy-induced intestinal mucositis by inhibiting the DNA damage in crypt cell in an extracellular signal-regulated kinase 1- and 2- dependent manner

Fig. 4

CIM model was induced by i.p. injection 5-FU (30 mg/kg/day) for 5 days. G-1 (0.03 mg/kg/day) and/or G15 (0.3 mg/kg/day) were administrated intraperitoneally with 5-FU together. G-1 was administrated alone or with G15 together in the CIM model to test the effect of GPER activation. On the 5th day, the ileum was collected for immunohistochemical staining and western blot. Data were expressed as mean ± SEM (*P < 0.05, ***P < 0.001). a Representative images for immunohistochemical staining of BrdU following G-1 administration with or without G15 in the CIM model (scale bars: 20 μm). b Representative images for immunohistochemical staining of Ki67 following G-1 administration with or without G15 in the CIM model (scale bars: 20 μm). c Statistical graph for the number of BrdU+ cells per crypt within four subgroups to show the effect of GPER activation on proliferation of crypt cells in the CIM model. At least 20 crypts were counted randomly for each sample, and the mean value was calculated for single sample (n = 6). d Statistical graph for the number of Ki67+ cells per crypt within four subgroups to show the effect of GPER activation on proliferation of crypt cells in the CIM model. At least 20 crypts were counted randomly for each sample, and the mean value was calculated for single sample (n = 6). e Statistical graph of villous height following G-1 administration with or without G15 in the CIM model. At least 20 villi were counted randomly for each sample, and the mean value was calculated for single sample (n = 10). f Statistical graph of crypt depth following G-1 administration with or without G15 in the CIM model. At least 20 crypts were counted randomly for each sample, and the mean value was calculated for single sample (n = 10). g Representative western blots photograph to show the effect of G-1 administration with or without G15 on cyclin D1 and cyclin B1 expression in the CIM model. h Statistical analysis of cyclin D1 and cyclin B1expression within four subgroups to show the effect of GPER activation on cyclin D1 and cyclin B1 expression in the CIM model (n = 5).

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