Fig. 1: SNS-032 inhibits the growth of ESCC cells. | Cell Death & Disease

Fig. 1: SNS-032 inhibits the growth of ESCC cells.

From: Transcriptional inhibition by CDK7/9 inhibitor SNS-032 suppresses tumor growth and metastasis in esophageal squamous cell carcinoma

Fig. 1

A Western blot analysis was performed to detect the protein levels of CDK7 and 9 in ESCC cells and Het-1A cells. B SNS-032 inhibited CTD phosphorylation of RNA Pol II in ESCC cells. Cells were treated with increasing concentration of SNS-032 for 48 h; immunoblotting analysis with the indicated antibodies was performed. C SNS-032 selectively inhibited the cell viability of ESCC cells examined by MTT assay. Five kinds of ESCC cells (KYSE30, KYSE150, KYSE450, KYSE510, and TE-1) and one immortalized esophageal epithelial cell line (Het-1A) were incubated with escalating concentrations of SNS-032 for 72 h, and then the cell viability was examined by MTT assay. D SNS-032 inhibited the anchorage-independent growth of ESCC cells. E Synergistic effect of the combinational treatment between SNS-032 and CDDP in ESCC cells (KYSE30 and KYSE150) when cell viability was assessed by MTS assay. CI (combinational index) values <1 indicate synergism between the two drugs. F, G After incubation with SNS-032 (0.25 μM) in combination with CDDP (10 μM) for 48 h, cells were collected and subjected to trypan blue exclusion assay (F) and western blot analysis (G). + indicates the presence and − indicates the absence of the indicated drugs. ***P < 0.001, by one-way ANOVA with post hoc intergroup comparison with Tukey’s test. H Simultaneously silencing CDK7 and CDK9 enhanced apoptosis and downregulation of Mcl-1 induced by CDDP in ESCC cells. I Combined SNS-032 and CDDP treatment synergistically inhibits tumor growth of ESCC in vivo. KYSE30 cells in nude mice were treated with SNS-032 at 7.5 mg/kg, CDDP at 5 mg/kg, or both for 4 weeks, as described in “Materials and methods.” Representative images of tumors from each group of mice are shown, and tumor weight was measured at the end of the experiment. *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA with post hoc intergroup comparison by Tukey’s test. J Western blot analysis of Mcl-1 in tumors tissues derived from SNS-032, CDDP, or both treated mice.

Back to article page