Fig. 3: Mcl-1 plays a critical role in SNS-032-induced apoptosis in ESCC cells. | Cell Death & Disease

Fig. 3: Mcl-1 plays a critical role in SNS-032-induced apoptosis in ESCC cells.

From: Transcriptional inhibition by CDK7/9 inhibitor SNS-032 suppresses tumor growth and metastasis in esophageal squamous cell carcinoma

Fig. 3

A Western blot analysis of the apoptosis-related protein expression in ESCC cells treated with SNS-032. B SNS-032 dose-dependently decreased mRNA levels of Mcl-1 in ESCC cells. C Overexpression of Mcl-1 abrogated the apoptosis induced by SNS-032. After transfection with the indicated plasmids (empty vector and Mcl-1) for 48 h, KYSE30 cells were treated with SNS-032 for 48 h, and then were detected by western blot analysis (top) and trypan blue exclusion assay. D Knocking down Mcl-1 sensitized the SNS-032-induced apoptosis. After transduction with the indicated siRNA (Mock, siMcl-1#1 and siMcl-1#2) for 48 h, KYSE30 cells were treated with SNS-032 for 48 h, and then were detected by western blot analysis (top) and trypan blue exclusion assay (bottom). *P < 0.05; **P < 0.01; ***P < 0.001, one-way ANOVA, post hoc intergroup comparisons, Tukey’s test.

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