Fig. 2: TDEVs released by GBM cells are taken up by T cells. | Cell Death & Disease

Fig. 2: TDEVs released by GBM cells are taken up by T cells.

From: CD73-positive extracellular vesicles promote glioblastoma immunosuppression by inhibiting T-cell clonal expansion

Fig. 2

A TDEVs in the cell culture supernatant of HAs and U-118 MG, U-87 MG and U-251 MG cells were obtained by ultrafiltration and characterised by transmission electron microscopy. B–E The count and size of TDEVs in the cell culture supernatant of HAs and U-118 MG, U-87 MG and U-251 MG cells were detected by nanoparticle tracking analysis. F Representative immunoblot of the microvesicle marker (ARF6), apoptotic body marker (THBS1), exosome markers (CD63 and TSG101), endoplasmic reticulum negative control marker (Erp72), lysosome negative control marker (Lamp1) and CD73 in cell lysates and TDEVs of HAs and U-118 MG, U-87 MG and U-251 MG cells. G Representative confocal microscopy images were used to visualise TDEV release within 6 h from HAs and the three GBM cell lines cotransfected with overexpression plasmids harbouring GFP-tagged CD73 and mCherry-tagged CD63. H Schematic diagram of the cocultivation experiment involving HACD73-GFP OE, U-118 MGCD73-GFP OE, U-87 MGCD73-GFP OE, U-251 MGCD73-GFP OE and T cells. I Representative confocal microscope images of GFP-labelled nanovesicles taken up by T cells cocultured with HAs and U-118 MG, U-87 MG and U-251 MG cells before and after 12 h. J Representative flow cytometry diagram of GFP intensity on the surface of T cells cocultured with HAs, U-118 MG cells, U-87 MG cells. Data are shown as the mean ± SD; t test, *p < 0.05 and **p < 0.01 compared to the GBM group. All data are from three independent experiments.

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