Fig. 2: CircRELL1 transport between cells could be mediated via exosomes. | Cell Death & Disease

Fig. 2: CircRELL1 transport between cells could be mediated via exosomes.

From: Exosomal circRELL1 serves as a miR-637 sponge to modulate gastric cancer progression via regulating autophagy activation

Fig. 2

A, B QRT-PCR analysis of the circRELL1 levels in the GES-1, AGS, SGC-7901, MKN45, and BGC-823 cells and their medium exosomes. C–E Transmission electron microscope (TEM), WB, and NTA of the exosomes derived from AGS medium (scale bar = 100 nm). F AGS cells transfected with GFP-circRELL1-overexpressing plasmid or vector were cocultured with AGS cells in a Transwell (membrane pore = 0.4 μm) plate. G A release of exosomes in AGS treated with GW4869 or DMSO, as Ach E activity assay determined. H Levels of circRELL1 in AGS treated with the medium following or not GW4869 treatment, as qRT-PCR determined. I, J Coculture of AGS and SGC-7901 with the exosomes for 6 and 24 h. Representative fluorescence images showing PKH26-labeled exosomes (red) and phalloidin-labeled F-actin (green) in the indicated cells counterstained with DAPI (blue) (scale bar: 20 μm). K Exosomes isolated from GC cell conditioning medium labeled with PKH26 (red) and transfected with GFP-circRELL1 (green) were cocultured with AGS and SGC-7901 cells for 24 h, while DAPI (blue) was used to stain nuclei (scale bar: 20 μm). L The efficiency of exosomes in delivering circRELL1 to AGS and SGC-7901 cells, as qRT-PCR analysis determined. Data indicate mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

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