Fig. 6: CircRELL1 acts as a miRNA sponge for miR-637. | Cell Death & Disease

Fig. 6: CircRELL1 acts as a miRNA sponge for miR-637.

From: Exosomal circRELL1 serves as a miR-637 sponge to modulate gastric cancer progression via regulating autophagy activation

Fig. 6

A qRT-PCR analysis was applied to measure subcellular localization of circRELL1 in GC cells. B Target miRNAs of circRELL1 were predicted by Circular RNA Interactome and circBank. C, D The relative miRNAs levels in the AGS and SGC-7901 cells after different treatments. E The predicted binding sites between circRELL1 and miR-637. F Dual-luciferase assay was performed to investigate the direct binding between circRELL1 and miR-637 in HEK-293 cells. G CircRELL1 were pulled down by biotin-labeled miR-637 and confirmed by qRT-PCR in AGS cells. H, I The assessment of the enrichment in Ago2 immunoprecipitates, as determined by WB and qRT-PCR. J qRT-PCR analysis of miR-637 levels in the paired GC and adjacent normal tissues. K Correlation analysis of the relationship between circRELL1 and miR-637 in 80 GC tissues. L FISH analysis of circRELL1 and miR-637 levels in GC and adjacent tissues. Results are presented as mean ± SD of three independent experiments. **P < 0.01, ***P < 0.001.

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