Fig. 3: Cell proliferation decline, apoptosis rising and cell cycle arrest accompanied with NUSAP1 silencing.

A Western blotting verification of CLL cells with NUSAP1 knockdown and recovered. B OD values of NUSAP1 knockdown CLL cells by CCK-8. C Test of apoptosis by flow cytometry in cells with NUSAP1 decrement and recovered. D The relative apoptotic rates in CLL cells with NUSAP1 knockdown and recovered. E Detection of cell cycle in cells with NUSAP1 decrement and recovered by flow cytometry assay. F The relative rates of cells in different cell cycle phases. All data are presented as the mean ± SD, n = 3. *p < 0.05; **p < 0.01.