Fig. 3: GPR120 had no effects on the activation, proliferation, and differentiation of CD4+ T cells in vitro. | Cell Death & Disease

Fig. 3: GPR120 had no effects on the activation, proliferation, and differentiation of CD4+ T cells in vitro.

From: GPR120 induces regulatory dendritic cells by inhibiting HK2-dependent glycolysis to alleviate fulminant hepatic failure

Fig. 3: GPR120 had no effects on the activation, proliferation, and differentiation of CD4+ T cells in vitro.The alternative text for this image may have been generated using AI.

T cells were isolated from the spleens of wild-type (WT) mice and GPR120−/− (GPR120 KO) mice. T cells were cultured (2 × 105 cells/well) in the presence of anti-CD3 and anti-CD28 antibodies. T cells from WT mice were treated with DMSO, TUG891 (20 μM) with or without AH7614 (20 μM). a, b The expression levels of CD25, CD44, CD62L, and CD69 on CD4+ T cells were analyzed by flow cytometry. c, d Frequencies of the proliferating (CFSElow) CD4+ T cell proliferation in response to the indicated treatments were measured by flow cytometry. e, f CD4+ T cells were stained for the surface marker CD4 and intracellular expression of TNF-α and IFN-γ on day 5 and analyzed by flow cytometry. g, h CD4+ T cells were stained for the surface marker CD4 and intracellular expression of Foxp3 on day 5 and analyzed by flow cytometry. The results are representative of three independent experiments and presented as the mean ± SEM. Significant differences were analyzed by One-way ANOVA. ns no significance.

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