Fig. 4: GPR120 regulated the activation and maturation of DCs, but had no effects on macrophage activation. | Cell Death & Disease

Fig. 4: GPR120 regulated the activation and maturation of DCs, but had no effects on macrophage activation.

From: GPR120 induces regulatory dendritic cells by inhibiting HK2-dependent glycolysis to alleviate fulminant hepatic failure

Fig. 4: GPR120 regulated the activation and maturation of DCs, but had no effects on macrophage activation.The alternative text for this image may have been generated using AI.

Wild-type (WT) and GPR120−/− (GPR120 KO) mice were injected with P. acnes (P.ac). Vehicle or TUG891 (10 mg/kg) was administered i.p. to WT mice on days 0, 2, 4, and 6 after P. acnes injection. Livers and peripheral blood were isolated from the mice (n = 7 mice per group). a Percentages of CD11c+ DCs in liver CD45+ cells and F4/80+ KCs in liver CD45+CD11b+ cells were analyzed by flow cytometry. b Absolute numbers of CD11c+ DCs and F4/80+ KCs in liver MNCs were analyzed by flow cytometry. c The percentages of splenic and liver DCs (CD11b and CD8 in the spleen) and (CD103 and CD11b in the liver) were analyzed by flow cytometry. d Levels of CD80, CD86, and MHC II on F4/80+ KCs and CD11c+ DCs in livers were analyzed by flow cytometry. e The percentages and numbers of CD11c+B220− DC precursors in the peripheral blood MNCs from each group were analyzed by flow cytometry. The results are representative of three independent experiments and presented as the mean ± SEM. Significant differences were analyzed by One-way ANOVA. **p < 0.01, ***p < 0.001, ns no significance.

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