Fig. 1: MICU3 was downregulated in skeletal muscle from old mice and senescent C2C12 cells induced by d-gal.
From: MICU3 regulates mitochondrial Ca2+-dependent antioxidant response in skeletal muscle aging
A IHC for MICU3 in mice gastrocnemius muscle (magnification ×400; scale bar = 20 μm). B, C Western blot and quantification of MICU3 and MCU protein level in mice gastrocnemius muscle. VDAC was used as the loading control. *indicates target bands. D The mRNA level of EMRE in mice gastrocnemius muscle. E, F Western blot and quantification for MICU3 in C2C12 cells. VDAC was used as the loading control. *indicates target bands. G SA-β-gal staining and quantification for C2C12 cells (scale bar = 50 μm). H Western blot and quantification for P16 and P53 in C2C12 cells. *indicates target bands. GAPDH was used as the loading control. Data were expressed as mean ± SEM, and data were analyzed using a one-way ANOVA. *p < 0.05 vs. the 6 M group; &p < 0.05, vs. The 18M group; n = 3 mice in each group; #p < 0.05, vs. the 0 g/L group; n = 3.
