Table 2 Primary antibodies used for western blotting and immunohistochemistry.
From: MICU3 regulates mitochondrial Ca2+-dependent antioxidant response in skeletal muscle aging
Antibody | Company (Lot.) | Working dilutions |
|---|---|---|
MICU3 | Sigma (R11856) | WB:1/250 IHC:1/150 |
SIRT1 | Abcam (ab32441) | WB:1/500 IHC:1/100 |
Desmin | Wanleibio (WL0174) | IF:1/200 |
MCU | Proteintech (26312-1-AP) | WB:1/2000 |
VDAC | Wanleibio (WL02790) | WB:1/500 |
PGC1α | Abcam (ab106814) | WB:1/500 |
Nrf-2 | ZENBIO (340675) | WB:1/1000 |
GAPDH | Proteintech (60004-1-lg) | WB:1/5000 |
MyoD | Santa Cruz (sc-71629) | WB:1/200 |
Myogenin | Santa Cruz (sc-52903) | WB:1/200 |
Cleaved Caspase-3 | Wanleibio (WL02117) | WB:1/500 |
Cleaved Caspase-9 | Wanleibio (WL01838) | WB:1/500 |
Caspase-9 | Wanleibio (WL03421) | WB:1/500 |
Caspase-3 | Wanleibio (WL04004) | WB:1/500 |
P16INK | Wanleibio (WLH3673) | WB:1/500 |
P53 | Abcam (ab26) | WB:1/1000 |
MyHC | Santa Cruz (sc-376157) | WB: 1/1000 IF: 1/200 |
