Fig. 3: Reduction of EZH2 activity enhances the aggressiveness of BLBC tumor cells. | Cell Death & Disease

Fig. 3: Reduction of EZH2 activity enhances the aggressiveness of BLBC tumor cells.

From: Decreased PRC2 activity supports the survival of basal-like breast cancer cells to cytotoxic treatments

Fig. 3

A Crystal violet staining of pG-2 cells with EZH2 knockdown. The confluency was measured by ImageJ and normalized to the controls. B Validation of EZH2 knockdown efficiency via qRT-PCR. Gene expression was calibrated to the control condition (siCont) and normalized to the Rplp0 housekeeping gene. C Measurement of EZH2 inhibition by increasing EPZ-6438 concentration via assessment of H3K27me3 levels in western blots. Densitometry normalized to actin levels is provided in the lower panel. D Proliferation assay of EPZ-6438-treated pG-2 cells using celigo® and crystal violet staining. E and F Colony formation assay upon treatment of pG-2 cells with EPZ-6438 alone (E) or in combination with CAF (F). The number of colonies was assessed through ImageJ analysis. Error bars: standard error of the mean (SEM). *p-val ≤ 0.05, **p-val ≤ 0.01, ***p-val ≤ 0.005. Student’s t-test: A and B one-way ANOVA: D–F All experiments were performed in biological triplicates (n = 3).

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