Fig. 4: Increased heterochromatin formation reduces proliferation of tumor cells from flies fed NDS. | Cell Death & Disease

Fig. 4: Increased heterochromatin formation reduces proliferation of tumor cells from flies fed NDS.

From: HP1a-mediated heterochromatin formation inhibits high dietary sugar-induced tumor progression

Fig. 4: Increased heterochromatin formation reduces proliferation of tumor cells from flies fed NDS.

Third instar eye discs of rasG12V; csk−/− and rasG12V, HP1a; csk−/− and rasG12V, HP1a-RNAi; csk−/− flies fed NDS or HDS with GFP-labeled tumor cells (green) and PH3 immunostaining (gray). Scale bar: 100 μm. A–A′′, D–D′′ rasG12V; csk−/−. B–B′′, E–E′′ rasG12V, HP1a; csk−/−. C–C′′, F–F′′ rasG12V, HP1a-RNAi; csk−/−. G Fluorescent quantification of PH3 puncta in tumor cells from rasG12V; csk−/− and rasG12V, HP1a; csk−/− and rasG12V, HP1a-RNAi; csk−/− flies fed NDS or HDS. All results analyzed and presented reflect data from three independent experiments (n = 15). Results are shown as mean ± SD of individual eye discs. Asterisk indicates a statistically significant difference (*p < 0.05). GFP green fluorescent protein, HDS high dietary sugar, NDS normal dietary sugar, NS not significant.

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