Fig. 6: Increased heterochromatin formation inhibits HDS-induced wingless expression in tumor cells.
From: HP1a-mediated heterochromatin formation inhibits high dietary sugar-induced tumor progression
Third instar eye discs of rasG12V; csk−/− and rasG12V, HP1a; csk−/− and rasG12V, HP1a-RNAi; csk−/− flies fed NDS or HDS with GFP-labeled tumor cells (green) and wingless staining (magenta). Scale bar: 100 μm. A–A′′, D–D′′ rasG12V; csk−/−. B–B′′, E–E′′ rasG12V, HP1a; csk−/−. C–C′′, F–F′′ rasG12V, HP1a-RNAi; csk−/−. G Quantification of wingless immunofluorescent signal in tumor cells of rasG12V; csk−/− and rasG12V, HP1a; csk−/− and rasG12V, HP1a-RNAi; csk−/− flies fed NDS or HDS. All results analyzed and presented reflect data from five independent experiments (n = 25). Results are shown as mean ± SD of individual eye discs. Asterisks indicate statistically significant differences via two-way ANOVA with relevant paired controls (*p < 0.05; **p < 0.01). GFP green fluorescent protein, HDS high dietary sugar, NDS normal dietary sugar, NS not significant.
