Fig. 1: MM01 is an inhibitor of ASC-mediated pro-Casp-1 activation.
From: Identification of an ASC oligomerization inhibitor for the treatment of inflammatory diseases
A Dose–response curve of compound MM01 (up to 40 µM) from an in vitro ASC-mediated pro-Casp-1 activation assay. B Recombinant caspase-1 activity was monitored in the presence of MM01 (10 µM) to exclude compounds affecting active caspase-1. An inhibitor of the caspase catalytic site, zVAD (20 nM), was used as a control. C Recombinant caspase-3 activity was measured in the presence of zVAD (20 nM) and MM01 (5 µM). D Recombinant caspase-9 activity was measured in the presence of zVAD (20 nM) and MM01 (5 µM). As an activation control, procaspase-9 incubated with Na-citrate buffer was used. E Apoptosome activity was monitored after reconstitution in vitro in the presence of zVAD (20 nM) and MM01 (5 µM). In all cases, data represent the mean ± SD of three independent experiments (***p < 0.001).
