Fig. 5: MM01 inhibits ASC-dependent inflammasome activity in stimulated murine peritoneal macrophages and in MSU-induced peritonitis mouse model.
From: Identification of an ASC oligomerization inhibitor for the treatment of inflammatory diseases
IL-1β (A), IL-6 (B), and TNF-α (D) secretion was evaluated by ELISA upon activation of the NLRP3 inflammasome with LPS (100 ng/ml) and ATP (2.5 mM) +/− MM01 at 1, 5, or 10 µM in isolated peritoneal macrophages. C Percentage of LDH release under the above-described conditions. In all cases, asterisks represent significant differences to the stimulated control (LPS/ATP), as determined by a one-way ANOVA with Tukey’s multiple comparisons test **p < 0.05; ***p < 0.001. All data expressed as mean ± SD of three experiments. E IL-1β detection by ELISA in the peritoneal cavity of C57BL/6 mice injected with MSU crystals with or without MM01 (10 mg/kg) treatment. F Neutrophil numbers in the peritoneal cavity of C57BL/6 mice treated as in the above-described conditions. Data are representative of two independent experiments (mean ± SD of n = 12). Asterisks represent significant differences to the stimulated control as determined by a one-way ANOVA with Tukey’s multiple comparisons test ***p < 0.001.
